diff --git a/sentry-android-core/src/main/java/io/sentry/android/core/AndroidOptionsInitializer.java b/sentry-android-core/src/main/java/io/sentry/android/core/AndroidOptionsInitializer.java
index 7817b5d6c6..5ce2746649 100644
--- a/sentry-android-core/src/main/java/io/sentry/android/core/AndroidOptionsInitializer.java
+++ b/sentry-android-core/src/main/java/io/sentry/android/core/AndroidOptionsInitializer.java
@@ -305,6 +305,28 @@ private static void setupProfiler(
       final @NotNull CompositePerformanceCollector performanceCollector) {
     if (options.isProfilingEnabled() || options.getProfilesSampleRate() != null) {
       options.setContinuousProfiler(NoOpContinuousProfiler.getInstance());
+      // Transaction-based profiling always relies on the legacy Debug-based profiler, so it is
+      // disabled together with legacy profiling. Perfetto profiling only supports continuous
+      // profiling.
+      if (!options.isEnableLegacyProfiling()) {
+        options
+            .getLogger()
+            .log(
+                SentryLevel.WARNING,
+                "Transaction-based profiling (profilesSampleRate/profilesSampler) is disabled "
+                    + "because enableLegacyProfiling is false. Transaction-based profiling always "
+                    + "uses the legacy profiler and is not supported by Perfetto. No profiling "
+                    + "data will be collected. Use profileSessionSampleRate for continuous "
+                    + "profiling instead.");
+        options.setTransactionProfiler(NoOpTransactionProfiler.getInstance());
+        if (appStartTransactionProfiler != null) {
+          appStartTransactionProfiler.close();
+        }
+        if (appStartContinuousProfiler != null) {
+          appStartContinuousProfiler.close(true);
+        }
+        return;
+      }
       // This is a safeguard, but it should never happen, as the app start profiler should be the
       // continuous one.
       if (appStartContinuousProfiler != null) {
diff --git a/sentry-android-core/src/test/java/io/sentry/android/core/AndroidOptionsInitializerTest.kt b/sentry-android-core/src/test/java/io/sentry/android/core/AndroidOptionsInitializerTest.kt
index 229d7ac2b6..855ee03267 100644
--- a/sentry-android-core/src/test/java/io/sentry/android/core/AndroidOptionsInitializerTest.kt
+++ b/sentry-android-core/src/test/java/io/sentry/android/core/AndroidOptionsInitializerTest.kt
@@ -424,6 +424,51 @@ class AndroidOptionsInitializerTest {
     assertEquals(fixture.sentryOptions.continuousProfiler, NoOpContinuousProfiler.getInstance())
   }
 
+  @Test
+  fun `init with profilesSampleRate and enableLegacyProfiling false noops both profilers`() {
+    fixture.initSut(
+      configureOptions = {
+        profilesSampleRate = 1.0
+        isEnableLegacyProfiling = false
+      }
+    )
+
+    assertEquals(NoOpTransactionProfiler.getInstance(), fixture.sentryOptions.transactionProfiler)
+    assertEquals(NoOpContinuousProfiler.getInstance(), fixture.sentryOptions.continuousProfiler)
+  }
+
+  @Test
+  fun `init with profilesSampler and enableLegacyProfiling false noops both profilers`() {
+    fixture.initSut(
+      configureOptions = {
+        profilesSampler = mock()
+        isEnableLegacyProfiling = false
+      }
+    )
+
+    assertEquals(NoOpTransactionProfiler.getInstance(), fixture.sentryOptions.transactionProfiler)
+    assertEquals(NoOpContinuousProfiler.getInstance(), fixture.sentryOptions.continuousProfiler)
+  }
+
+  @Test
+  fun `init with profilesSampleRate and enableLegacyProfiling false closes app start profiler`() {
+    val appStartProfiler = mock<ITransactionProfiler>()
+    AppStartMetrics.getInstance().appStartProfiler = appStartProfiler
+    fixture.initSut(
+      configureOptions = {
+        profilesSampleRate = 1.0
+        isEnableLegacyProfiling = false
+      }
+    )
+
+    assertEquals(NoOpTransactionProfiler.getInstance(), fixture.sentryOptions.transactionProfiler)
+    verify(appStartProfiler).close()
+
+    // AppStartMetrics should be cleared
+    assertNull(AppStartMetrics.getInstance().appStartProfiler)
+    assertNull(AppStartMetrics.getInstance().appStartContinuousProfiler)
+  }
+
   @Test
   fun `init reuses transaction profiler of appStartMetrics, if exists`() {
     val appStartProfiler = mock<ITransactionProfiler>()
diff --git a/sentry/src/main/java/io/sentry/SentryOptions.java b/sentry/src/main/java/io/sentry/SentryOptions.java
index 5340e20c6e..db10830c05 100644
--- a/sentry/src/main/java/io/sentry/SentryOptions.java
+++ b/sentry/src/main/java/io/sentry/SentryOptions.java
@@ -649,8 +649,8 @@ public class SentryOptions {
   private boolean startProfilerOnAppStart = false;
 
   /**
-   * When false, the legacy {@code Debug}-based profiler is disabled on API < 35 devices. On API 35+
-   * devices, Android's {@code ProfilingManager} (Perfetto-based stack sampling) is always used
+   * When false, the legacy {@code Debug}-based profiler is disabled on API &lt; 35 devices. On API
+   * 35+ devices, Android's {@code ProfilingManager} (Perfetto-based stack sampling) is always used
    * regardless of this setting. This option will be deprecated in the next major release and
    * removed in the one after.
    */
@@ -2247,9 +2247,13 @@ public void setStartProfilerOnAppStart(final boolean startProfilerOnAppStart) {
   }
 
   /**
-   * Whether the legacy {@code Debug}-based profiler is enabled on API < 35 devices. On API 35+,
-   * Android's {@code ProfilingManager} (Perfetto) is always used regardless of this setting. This
-   * option will be deprecated in the next major release and removed in the one after.
+   * Whether the legacy {@code Debug}-based profiler is enabled. This controls continuous profiling
+   * on API &lt; 35 devices (on API 35+, Android's {@code ProfilingManager} / Perfetto is always
+   * used for continuous profiling regardless of this setting) as well as transaction-based
+   * profiling ({@code profilesSampleRate}/{@code profilesSampler}) on all devices, since
+   * transaction-based profiling always relies on the legacy profiler and is not supported by
+   * Perfetto. This option will be deprecated in the next major release and removed in the one
+   * after.
    *
    * @return true if legacy profiling is enabled (default).
    */
@@ -2258,12 +2262,15 @@ public boolean isEnableLegacyProfiling() {
   }
 
   /**
-   * Set whether the legacy {@code Debug}-based profiler is enabled on API < 35 devices. Set to
-   * {@code false} to disable profiling on devices below API 35. On API 35+ devices, Android's
-   * {@code ProfilingManager} (Perfetto) is always used and this setting has no effect. This option
-   * will be deprecated in the next major release and removed in the one after.
+   * Set whether the legacy {@code Debug}-based profiler is enabled. Set to {@code false} to disable
+   * continuous profiling on devices below API 35 (on API 35+ devices, Android's {@code
+   * ProfilingManager} / Perfetto is always used for continuous profiling and this setting has no
+   * effect) as well as transaction-based profiling ({@code profilesSampleRate}/{@code
+   * profilesSampler}) on all devices, since transaction-based profiling always relies on the legacy
+   * profiler and is not supported by Perfetto. This option will be deprecated in the next major
+   * release and removed in the one after.
    *
-   * @param enableLegacyProfiling false to disable legacy profiling on API < 35.
+   * @param enableLegacyProfiling false to disable legacy profiling.
    */
   public void setEnableLegacyProfiling(final boolean enableLegacyProfiling) {
     this.enableLegacyProfiling = enableLegacyProfiling;